The 5 health and fitness care workers ended up definitively seronegative towards H5 in all assays, which means they weren't contaminated with H5. This analyze demonstrates a pattern of weakening binding affinity of present antibodies from H5 isolates after a while, indicating that the H5N1 virus is evolving immune escape https://www.dailypioneer.com/2014/state-editions/anganwadi-supervisors-get-another-chance-for-document-verification.html